Xenbase Image ID: 135228
Figure 5. Injection of CSKA-X8 DNA into dorsal blastomeres
of Xenopus embryos results in loss of forebrain and notochord.
(A-F) Transverse sections taken at a comparable distance from
the anterior end of CSKA/CAT (A-C}- or CSKA/X8 (D-F)-injected
embryos. (G,H) Sagittal sections through CSKA/CAT
(G)- or CSKA/X8 (H)-injected embryos. Abbreviations are as follows:
(pros) Prosencephalon (forebrain); (soml somitic mesoderm
(muscle); (not) notochord; (sc) spinal cord; (fp) floor plate
cells of the ventral spinal cord; (rhom) rhombencephalon (hindbrain);
(ot} otic vessicle (ear anlage); (vsc) ventral wall of the
spinal cord. Bar in A represents 400 t~m in all panels except for
C and F, where it represents 150 ~m. (/, J) Notochord immunostaining
in stage 28 (I) or stage 21 (J) neurulae using monoclonal
antiserum Tor70. Open arrows denote normal notochord staining
in CSKA/CAT-injected (upper) embryos; solid arrows in I
indicate anterior and (faint) posterior notochord staining of this
CSKA/X8-injected embryo. Staining is not detected between
the two arrows in the mid-body region. The lower embryo in J
represents a CSKA/X8-injected embryo in which notochord
staining is absent.
Image published in: Christian JL and Moon RT (1993)
Copyright © 1993. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article.
Printer Friendly View