XB-IMG-135228
Xenbase Image ID: 135228
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Figure 5. Injection of CSKA-X8 DNA into dorsal blastomeres
of Xenopus embryos results in loss of forebrain and notochord.
(A-F) Transverse sections taken at a comparable distance from
the anterior end of CSKA/CAT (A-C}- or CSKA/X8 (D-F)-injected
embryos. (G,H) Sagittal sections through CSKA/CAT
(G)- or CSKA/X8 (H)-injected embryos. Abbreviations are as follows:
(pros) Prosencephalon (forebrain); (soml somitic mesoderm
(muscle); (not) notochord; (sc) spinal cord; (fp) floor plate
cells of the ventral spinal cord; (rhom) rhombencephalon (hindbrain);
(ot} otic vessicle (ear anlage); (vsc) ventral wall of the
spinal cord. Bar in A represents 400 t~m in all panels except for
C and F, where it represents 150 ~m. (/, J) Notochord immunostaining
in stage 28 (I) or stage 21 (J) neurulae using monoclonal
antiserum Tor70. Open arrows denote normal notochord staining
in CSKA/CAT-injected (upper) embryos; solid arrows in I
indicate anterior and (faint) posterior notochord staining of this
CSKA/X8-injected embryo. Staining is not detected between
the two arrows in the mid-body region. The lower embryo in J
represents a CSKA/X8-injected embryo in which notochord
staining is absent. Image published in: Christian JL and Moon RT (1993) Copyright © 1993. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |