Figure 5. Injection of CSKA-X8 DNA into dorsal blastomeres of Xenopus embryos results in loss of forebrain and notochord. (A-F) Transverse sections taken at a comparable distance from the anterior end of CSKA/CAT (A-C}- or CSKA/X8 (D-F)-injected embryos. (G,H) Sagittal sections through CSKA/CAT (G)- or CSKA/X8 (H)-injected embryos. Abbreviations are as follows: (pros) Prosencephalon (forebrain); (soml somitic mesoderm (muscle); (not) notochord; (sc) spinal cord; (fp) floor plate cells of the ventral spinal cord; (rhom) rhombencephalon (hindbrain); (ot} otic vessicle (ear anlage); (vsc) ventral wall of the spinal cord. Bar in A represents 400 t~m in all panels except for C and F, where it represents 150 ~m. (/, J) Notochord immunostaining in stage 28 (I) or stage 21 (J) neurulae using monoclonal antiserum Tor70. Open arrows denote normal notochord staining in CSKA/CAT-injected (upper) embryos; solid arrows in I indicate anterior and (faint) posterior notochord staining of this CSKA/X8-injected embryo. Staining is not detected between the two arrows in the mid-body region. The lower embryo in J represents a CSKA/X8-injected embryo in which notochord staining is absent.
Image published in: Christian JL and Moon RT (1993)
Copyright © 1993. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article.
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