Xenbase Image ID: 140029
Figure 3. nodal1 and gdf3 Cooperate in the
Regulation of Cilia Length
(A) nodal1 and gdf3 expression in the area of
Notch1 morphant GRPs at stage 13/14 by wholemount
in situ hybridization. Red arrowheads indicate
reduction of nodal1 and gdf3 expression.
A white triangle indicates the central area of the
GRP. ‘‘n’’ indicates number of analyzed embryos.
a, anterior; p, posterior; L, left; R, right.
(B) Shortened and rescued cilia at the GRP.
Xnr1MO (10 ng), DerMO (10 ng), and/or conMO
(20 ng) were injected. The scale bar represents
20 mm. a, anterior; p, posterior.
(C) Average length of cilia from (B).
(D and E) Leftward flow after single and double
knockdown of nodal1 and gdf3. Velocity (D) is
shown in mm/s, and directionality (E) is shown in
rho. Rhodamine-B dextran was used as a lineage
Statistical analyses were carried out using Excel
using SEM and Student’s t test to calculate
p values (*p % 0.05, **p % 0.005, ***p % 0.0005,
****p % 0.00005; n.s., not significant).
Image published in: Tözser J et al. (2015)
Copyright © 2015. Image reproduced on Xenbase with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
Image source: Published
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