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Figure 3. nodal1 and gdf3 Cooperate in the Regulation of Cilia Length (A) nodal1 and gdf3 expression in the area of Notch1 morphant GRPs at stage 13/14 by wholemount in situ hybridization. Red arrowheads indicate reduction of nodal1 and gdf3 expression. A white triangle indicates the central area of the GRP. ‘‘n’’ indicates number of analyzed embryos. a, anterior; p, posterior; L, left; R, right. (B) Shortened and rescued cilia at the GRP. Xnr1MO (10 ng), DerMO (10 ng), and/or conMO (20 ng) were injected. The scale bar represents 20 mm. a, anterior; p, posterior. (C) Average length of cilia from (B). (D and E) Leftward flow after single and double knockdown of nodal1 and gdf3. Velocity (D) is shown in mm/s, and directionality (E) is shown in rho. Rhodamine-B dextran was used as a lineage tracer. Statistical analyses were carried out using Excel using SEM and Student’s t test to calculate p values (*p % 0.05, **p % 0.005, ***p % 0.0005, ****p % 0.00005; n.s., not significant).

Image published in: Tözser J et al. (2015)

Copyright © 2015. Image reproduced on Xenbase with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

nodal1.L nr1, Xnr-1, Xnr1, nodal-related 1, nr1-a, spaw, nodalX.laevisSometime during NF stage 13 to NF stage 14gastrocoel roof plate
gdf3.L derrière, derriere, vgr2, vgr-2, gdf-3X.laevisSometime during NF stage 13 to NF stage 14gastrocoel roof plate
tuba4b.L tuba4, alpha tubulin, alpha-tubulin, tuba4aX.laevisSometime during NF stage 13 to NF stage 14gastrocoel roof plate
ciliated cell

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