Xenbase Image ID: 150106

Figure S1: Validation of pSmad1/5/8 staining in response to BMP manipulation. (A): Injection of stage 9 Xenopus embryos with recombinant BMP4 led to increased Smad1/5/8 phosphorylation and nuclear localization. (B): The percentage of nuclei positive for the pSmad signal is significantly higher in BMP-treated than in control embryos (Student's t-test). (C): Knocking down BMP2, BMP4 and BMP7 with specific morpholino oligonucleotides inhibited Smad1/5/8 phosphorylation. Embryos were injected with either GFP mRNA alone (upper row) or GFP mRNA and the BMP2/4/7 MOs (lower row). The pSmad1/5/8 signal was lost in cells that received the BMP2/4/7 MOs, but not in those that received only the GFP mRNA. Image published in: Cibois M et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue smad1.L MLP, smad-1, smad1-a, smad1-b, XMad, Xmad1, Xsmad1 X. laevis Sometime during NF stage 9 to NF stage 10 ectoderm nucleus smad9.L smad8, smad8a, smad8b, xsmad9 X. laevis Sometime during NF stage 9 to NF stage 10 ectoderm nucleus Image source: Published Larger Image Printer Friendly View

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