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Figure S1: Validation of pSmad1/5/8 staining in response to BMP manipulation. (A): Injection of stage 9 Xenopus embryos with recombinant BMP4 led to increased Smad1/5/8 phosphorylation and nuclear localization. (B): The percentage of nuclei positive for the pSmad signal is significantly higher in BMP-treated than in control embryos (Student's t-test). (C): Knocking down BMP2, BMP4 and BMP7 with specific morpholino oligonucleotides inhibited Smad1/5/8 phosphorylation. Embryos were injected with either GFP mRNA alone (upper row) or GFP mRNA and the BMP2/4/7 MOs (lower row). The pSmad1/5/8 signal was lost in cells that received the BMP2/4/7 MOs, but not in those that received only the GFP mRNA.

Image published in: Cibois M et al. (2015)

Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

smad1.LXsmad1, smad-1, Xmad1, MLP, XMad, smad1-a, smad1-bX.laevisSometime during NF stage 9 to NF stage 10ectoderm
smad9.Lxsmad9, smad8, smad8a, smad8bX.laevisSometime during NF stage 9 to NF stage 10ectoderm

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