Xenbase Image ID: 156906

Fig. 5. Neural patterning and differentiation are disrupted upon pou5f3 overexpression. Injection of individual pou5f3 mRNAs causes changes in neural marker gene expression, as assayed by whole-mount RNA in situ hybridization at stages 16 (A-T) and 23 (U-Y) using markers otx2, krox20, and hoxb9 in combination (A-D), pax3 (F-I), pax6 (K-N), sox2 (p-S), and tubb2b (U-X); dorsal view, anterior up. Asterisks in images indicate injected side; arrows indicate partial loss of expression; arrowheads indicate posterior shifts in expression. Quantification (E, J, O, T, and Y) shows the percentage of embryos with symmetrical (white bars) or asymmetrical (grey bars) neural gene expression. n >30 embryos per experimental condition; **, p<0.01, *** p<0.001 by pairwise chi-squared test. Image published in: Exner CRT et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue otx2.L otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 16 anterior egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Throughout NF stage 16 rhombomere R3 rhombomere R5 hoxb9.L hox 2.5, xhoxb9, XlHbox-6, XlHbox6 X. laevis Throughout NF stage 16 posterior pax3.L cdhs, hup2, pax-3, pax3-a, pax3-b, Waardenburg syndrome 1, ws1, xpax3 X. laevis Throughout NF stage 16 neural plate neural plate border pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 16 neural plate anterior neural ridge sox2.S anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 16 neural plate tubb2b.L N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 23 neuroectoderm Image source: Published Larger Image Printer Friendly View

Return to previous page