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Fig. 5. Neural patterning and differentiation are disrupted upon pou5f3 overexpression. Injection of individual pou5f3 mRNAs causes changes in neural marker gene expression, as assayed by whole-mount RNA in situ hybridization at stages 16 (A-T) and 23 (U-Y) using markers otx2, krox20, and hoxb9 in combination (A-D), pax3 (F-I), pax6 (K-N), sox2 (p-S), and tubb2b (U-X); dorsal view, anterior up. Asterisks in images indicate injected side; arrows indicate partial loss of expression; arrowheads indicate posterior shifts in expression. Quantification (E, J, O, T, and Y) shows the percentage of embryos with symmetrical (white bars) or asymmetrical (grey bars) neural gene expression. n >30 embryos per experimental condition; **, p<0.01, *** p<0.001 by pairwise chi-squared test.

Image published in: Exner CRT et al. (2017)

Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V.

otx2.LXotx2, otx-2, Xotx-2, otxA, otx2-a, otx2-bX.laevisThroughout NF stage 16anterior
egr2.LXKr20, EGR-2, Krox-20, XKrox-20, krox20X.laevisThroughout NF stage 16rhombomere R3
rhombomere R5
hoxb9.Lxhoxb9, hox 2.5, XlHbox-6, XlHbox6X.laevisThroughout NF stage 16posterior
pax3.Lpax-3, Waardenburg syndrome 1, xpax3, pax3-a, pax3-b, ws1, cdhs, hup2X.laevisThroughout NF stage 16neural plate
neural plate border
pax6.LXLPAX6, xpax6, pax-6, an2, mgda, wagr, pax6-a, pax6-bX.laevisThroughout NF stage 16neural plate
anterior neural ridge
sox2.SXLSOX-2, Sox-2, XSox2, Xsox-2, anop3, mcops3X.laevisThroughout NF stage 16neural plate
tubb2b.Ln-tubulin, ntubulin, Xn-tubulin, N-Tub, neural beta-tubulin, NBT, NSTX.laevisThroughout NF stage 23neuroectoderm

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