Fig. 5. Neural patterning and differentiation are disrupted upon pou5f3 overexpression. Injection of individual pou5f3 mRNAs causes changes in neural marker gene expression, as assayed by whole-mount RNA in situ hybridization at stages 16 (A-T) and 23 (U-Y) using markers otx2, krox20, and hoxb9 in combination (A-D), pax3 (F-I), pax6 (K-N), sox2 (p-S), and tubb2b (U-X); dorsal view, anterior up. Asterisks in images indicate injected side; arrows indicate partial loss of expression; arrowheads indicate posterior shifts in expression. Quantification (E, J, O, T, and Y) shows the percentage of embryos with symmetrical (white bars) or asymmetrical (grey bars) neural gene expression. n >30 embryos per experimental condition; **, p<0.01, *** p<0.001 by pairwise chi-squared test.
Image published in: Exner CRT et al. (2017)
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