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Fig 2. Test crosses between animals carrying tyr-mutated leapfrog transplants and albinos demonstrate germline transmission of mutant alleles. (A) Leapfrog transplant-bearing male (pigmented) is shown amplexed with an albino tyr−/− female. (B) Leapfrog transplant-bearing female (pigmented) is shown amplexed with an albino tyr−/− male. (C) Examples of F1 progeny from the cross in A grown to tadpole stage. These tadpoles are albino because they inherited tyr mutant alleles from both F0 parents. Therefore the leapfrog-generated frog carries gametes derived from CRISPR-mutated PGCs. The inset in C shows an unrelated pigmented tadpole at roughly the same stage for comparison. Image published in: Blitz IL et al. (2016) Copyright © 2016. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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