Xenbase Image ID: 175542
Figure 4: Kmt2d knockdown does not affect heart rate of Xenopus tailbud
stage embryos. Embryos were injected with 2.5 ng MO Kmt2d (or 2.5 ng 5 bp
mismatch MO) or 5 ng splice MO (or 5 ng control MO) in one dorsal blastomere at
the 4-cell stage. 50 pg mGFP mRNA were co-injected as a lineage tracer. The
beating frequency of the embryos’ hearts was analyzed at stage 44. Representative
embryos (A-E) are shown from the ventral side; the respective heart rate is indicated.
Asterisks mark the injected side. A'-E' show a higher magnification of the heart area.
Ventricle and outflow tract are indicated by dashed circles. Movies of the beating
hearts of the presented embryos can be found in the supplement. A,B,C Uninjected
(A,A') as well as mismatch MO (mis) (B,B') and control MO (coMO) (C,C') injected
embryos showing a normal heart morphology. D,E Embryos injected with Kmt2d MO
(MO) (D,D') and splice MO (spMO) (E,E') displaying reduced ventricle size. F Boxplot
showing the heart rates of 30 embryos per condition from two independent
Image published in: Schwenty-Lara J et al. (2019)
Copyright © 2019. Image reproduced with permission of the Publisher, John Wiley & Sons.
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