Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.

Xenbase Image ID: 175542

Figure 4: Kmt2d knockdown does not affect heart rate of Xenopus tailbud stage embryos. Embryos were injected with 2.5 ng MO Kmt2d (or 2.5 ng 5 bp mismatch MO) or 5 ng splice MO (or 5 ng control MO) in one dorsal blastomere at the 4-cell stage. 50 pg mGFP mRNA were co-injected as a lineage tracer. The beating frequency of the embryos’ hearts was analyzed at stage 44. Representative embryos (A-E) are shown from the ventral side; the respective heart rate is indicated. Asterisks mark the injected side. A'-E' show a higher magnification of the heart area. Ventricle and outflow tract are indicated by dashed circles. Movies of the beating hearts of the presented embryos can be found in the supplement. A,B,C Uninjected (A,A') as well as mismatch MO (mis) (B,B') and control MO (coMO) (C,C') injected embryos showing a normal heart morphology. D,E Embryos injected with Kmt2d MO (MO) (D,D') and splice MO (spMO) (E,E') displaying reduced ventricle size. F Boxplot showing the heart rates of 30 embryos per condition from two independent experiments.

Image published in: Schwenty-Lara J et al. (2019)

Copyright © 2019. Image reproduced with permission of the Publisher, John Wiley & Sons.

Larger Image
Printer Friendly View

Return to previous page
Xenbase: The Xenopus Model Organism Knowledgebase.
Version: 4.14.0
Major funding for Xenbase is provided by grant P41 HD064556