Figure 4: Kmt2d knockdown does not affect heart rate of Xenopus tailbud stage embryos. Embryos were injected with 2.5 ng MO Kmt2d (or 2.5 ng 5 bp mismatch MO) or 5 ng splice MO (or 5 ng control MO) in one dorsal blastomere at the 4-cell stage. 50 pg mGFP mRNA were co-injected as a lineage tracer. The beating frequency of the embryos’ hearts was analyzed at stage 44. Representative embryos (A-E) are shown from the ventral side; the respective heart rate is indicated. Asterisks mark the injected side. A'-E' show a higher magnification of the heart area. Ventricle and outflow tract are indicated by dashed circles. Movies of the beating hearts of the presented embryos can be found in the supplement. A,B,C Uninjected (A,A') as well as mismatch MO (mis) (B,B') and control MO (coMO) (C,C') injected embryos showing a normal heart morphology. D,E Embryos injected with Kmt2d MO (MO) (D,D') and splice MO (spMO) (E,E') displaying reduced ventricle size. F Boxplot showing the heart rates of 30 embryos per condition from two independent experiments.
Image published in: Schwenty-Lara J et al. (2019)
Copyright © 2019. Image reproduced with permission of the Publisher, John Wiley & Sons.
| Experiment + Assay | Source | Phenotypes and Disease | ||||
|---|---|---|---|---|---|---|
| Xla Wt + kmt2d MO + NF44 (whole-mount microscopy) | Fig. 4 D D' |
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| Xla Wt + kmt2d MO + NF44 (whole-mount microscopy) | Fig. 4 E E' |
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