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FIGURE S1 | Expression patterns for WHS related genes across early development. In situ hybridization utilized (A–F) antisense mRNA probe to whsc1, (G–L) full-length antisense mRNA probe to whsc2, (M–R) full-length antisense mRNA probe to letm1, and (S-X) 1 kb partial-length antisense mRNA probe to tacc3. Embryos shown at blastula stage (A,G,M,S), in dorsal view at stage 16–20 (B,H,N,T), in lateral view at stage 20–25 (C,I,O,U), detail of lateral anterior region at stage 35 (D,J,P,V), and in both lateral and dorsal views from stages 39–42 (E,K,Q,W and F,L,R,X). (Y) In situ hybridization probes generated against sense strands of WHS gene mRNAs, shown at stage 25. Brown coloration (S,T) is unbleached pigment, unrelated to in situ hybridization staining. Scalebar is 250 μm.bryos were anesthetized with benzocaine.
Image published in: Mills A et al. (2019)
Copyright © 2019. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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