XB-IMG-83135
Xenbase Image ID: 83135
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Fig. 2. Localization and transcriptional
regulation of bowline
transcripts. (A-C) Xenopus
embryos from different
stages were stained for expression
of bowline mRNA by
whole-mount in situ hybridization
at (A) stage 13, (B) stage
18 and (C) stage 30. Embryos
are oriented in dorsal view with
anterior to the top except for
(C), for which the anterior is to
the left. (D-I) Longitudinal section
through stage 20 embryos
stained for expression of XDelta-
2 (D), bowline (E,G,I) and
Thy1 (F,H). Embryos are oriented
with anterior to the left.
Arrowheads on the lower side
of the embryos indicate the
anterior end of X-Delta-2-and
Thy1-expressing regions. The
probes used are shown in the
upper right corner. In (H and I),
the width of the expression region
for Thy1 and bowline are
indicated as red and black lines,
respectively. (J) Diagram showing
the position of bowline expression
relative to that of other
genes known to be expressed
segmentally in X. laevis embryos (Jen et al., 1997, Kim et al., 2000, Sparrow et al., 1998). (KM)
Embryos were unilaterally injected with β-gal RNA (K), XotchδE RNA (L), or XSu(H)DBM
RNA (M) and analyzed for the expression of bowline (K,L,M) by whole-mount in situ
hybridization at stage 20. RNA encoding the lineage tracer β-gal was co-injected to identify
the injected side (red staining). Dorsal views with anterior towards the top are shown.
Injected sides are indicated as �inj.� Somitomeres were demarcated as S0, S-I, S-II, S-III
based on the annotation of Pourqui� and Tam (Pourqui� and Tam, 2001). Bars, 100 μm. Image published in: Kondow A et al. (2006) Copyright © 2006. Reproduced with permission of the Publisher, University of the Basque Country Press.
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