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Figure 8. XE2F Lipofection Inhibits Xotx2 Translation and the Generation of Bipolar Cells(A–C) st. 40-lipofected retinas. Lipofected cells are traced by GFP (green), Xotx2 immunostaining in red. Arrows indicate Xotx2-positive lipofected cells.(D) Histogram showing the proportion of retinal lipofected cells expressing Xotx2 protein. Number of counted cells is indicated by n. Double asterisk indicates p = 0.009; triple asterisk indicates p = 0.0001 (student's t-test); error bars indicate standard error of the mean.(E–G) Cell type analysis of lipofected retinas at st. 42. Examples are shown of retinas lipofected with cdk2/cyclinA2 (cdk/cyc in [E]) and XE2F (F). GCL: ganglion cell layer; INL: inner nuclear layer; ONL: outer nuclear layer; pe, pigmented epithelium. Red arrowheads point at cells with amacrine morphology, which represent the majority of cell types in the INL of XE2F-lipofected retinas. Bars in (G) show the proportion of lipofected cells types at st. 42. Number of counted cells is indicated by n; double asterisk indicates p ≤ 0.01; triple asterisk indicates p ≤ 0.001 (student's t-test); error bars: standard error of the mean. The lipofection of cdk2/cyclinA2 and XE2F increases and decreases, respectively, the proportion of bipolar cells compared to control. The decrease of photoreceptors after cdk2/cyclinA2 lipofection is due to a reduction of cones [18].

Image published in: Decembrini S et al. (2006)

Image downloaded from an Open Access article in PubMed Central. Copyright: © 2006 Decembrini et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

GeneSynonymsSpeciesStage(s)Tissue
otx2.LXotx2, otx-2, Xotx-2, otxA, otx2-a, otx2-bX.laevisThroughout NF stage 40retina
bipolar neuron

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