Figure 6. Depletion of xWRN reduces SSA. (A) Western blot of the depleted NPE. The three lanes on the right are quantitation controls and contain normal NPE at 1%, 2%, and 10% of the amount loaded in the lanes containing the depleted NPE. (B) SSA assay with xWRN-depleted and mock-depleted NPE. pRW4′ was incubated in NPE for the indicated times, treated with SDS/proteinase K, and separated by agarose gel electrophoresis. The SSA products include the band indicated by the arrow and a subset of the bands indicated by the bracket. (C) Staining intensity plot of the lanes containing the 2-h repair products in (B).
Image published in: Yan H et al. (2005)
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