Figure 2. HSET predominately localizes between parallel microtubules in the mitotic spindle of human CF-PAC-1 cells. Cultured CF-PAC-1 cells were fixed and processed for immunogold EM as described in Materials and Methods. A, Low magnification image of a cell processed for EM under these conditions. Bar, 1 μm. B–D, High magnification images showing typical HSET localization between microtubules within the spindle. Frequently, HSET localized to microtubules that terminated within a mass of chromatin (B and C; black star) or within a kinetochore (D; arrow). Arrowhead in D indicates a gold particle. Bars, 0.1 μm. E, Sections through the half spindle of four independent cells were divided into 1-μm regions perpendicular to the long axis of the spindle, with the first region (1 μm) spanning the centrosome and the last region (6 μm) close to the chromosomes. The total number of microtubules and gold particles (generated by immunolabeling for HSET) were counted in each section. The total values were averaged over the number of sections, and the average number of gold particles and microtubules plotted as a function of the region of the spindle.
Image published in: Mountain V et al. (1999)
© 1999 The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license
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