Figure 3. The effect of FFA-1 depletion on DNA replication and RPA focus formation. (A) Western blot for FFA-1 in the extracts depleted with either anti–FFA-1 antibody or control antibody. Arrowhead indicates the position of FFA-1. (B) DNA synthesis in nuclei reconstituted around sperm chromatin with the depleted extracts. Samples were taken at the times indicated. (C and D) Immunofluorescence staining for RPA on sperm chromatin incubated in either FFA-1–depleted (C) or control-depleted cytosol (D).
Image published in: Chen CY et al. (2001)
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