Figure 4. Cep152 is required for centriole duplication and amplification. (A) Western blotting of U2OS cell lysates 72 h after transfection with control (Ctl) siRNAs or Cep152 siRNAs immunoblotted for Cep152 or α-tubulin as a loading control. The indicated Cep152 protein levels are normalized to α-tubulin levels. Black lines indicate that intervening lanes have been spliced out. (B) U2OS cells were fixed at the indicated times after Cep152 siRNA transfection, and centriole number was determined by labeling for centrin and γ-tubulin. Insets show enlarged centrosomes. (C) Quantification of B. Centriole number in cells transfected with control siRNAs for 96 h was determined as described above. n = 200 cells over three experiments for each time point. (D) RPE-1 cells bearing Tet-inducible Plk4 were depleted of Cep152 by siRNA for 72 h then treated with doxycycline for 18 h to induce Plk4 expression. Centriole number was determined by labeling for centrin, and depleted cells were identified by reduced Cep152 staining. (B and D) Insets show enlarged centrosomes. Bars, 5 µm.
Image published in: Hatch EM et al. (2010)
© 2010 Hatch et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license
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