Figure 6. DSB repair in human fibroblasts upon G6PD silencing. (A) Cells were transfected with a scrambled siRNA (ctr) and G6PD targeting siRNA. An aliquot of the cells was collected, lysed and analysed by western blot anti-G6PD (upper panel) and α-tubulin (bottom panel). (B) Another aliquot of the cells was either left untreated or irradiated with 10 Gy. Residual DSBs remaining after the indicated times were determined by neutral comet assay. The histogram represents the average comet tail moment. Experiment was repeated three times. Error bars represent s.d. (C) Cells transfected with control or G6PD-specific siRNA were exposed to 2 Gy of IR. γH2AX foci were visualized by immunofluorescence. (D) The histogram represents number of foci per cells. The data are the average of three independent experiments. Error bars indicate s.d.
Image published in: Cosentino C et al. (2011)
Copyright © 2011, European Molecular Biology Organization. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license
Permanent Image Page
Printer Friendly View
XB-IMG-125732