Figure 1. MiR-124 regulates cell proliferation and neurogenesis in the optic vesicle and forebrain. (A) Proliferating cells were detected with a BrdU (red) incorporation assay. Hoechst (blue) was applied to label the nuclei of all cells. The dashed line in the schematic diagram [images from Nieuwkoop and Faber, 1994, Normal Table of Xenopus laevis (Daudin)] indicates the location of the transverse sections in the developing eye. In the optic vesicle (arrow) and forebrain (arrow head) of embryos at stage (st.) 22/23, the BrdU-positive cell ratio was significantly reduced when a miR-124 inhibitor (Anti-124) was injected, but significantly increased when an miR-124 precursor (Pre-124) was applied. In the optic cup (st.33/34), injection of either control inhibitor (Anti-ctrl) or precursor (Pre-ctrl) molecules gave no significant change in cell proliferation compared with the uninjected control (Uninj). The bar graph illustrates the BrdU-positive ratio of the transverse sections (mean ± SEM, 24 sections from six embryos). Scale bar: 100 μm. (B) Expression of NCAM and N-tubulin are significantly upregulated with downregulation of miR-124, but significantly downregulated with overexpression of miR-124. ODC and –RT are the internal and negative controls, respectively, for the RT–PCR procedure. The bar graph illustrates the gene expression level analyzed by real-time RT–PCR. Means ± SEM are from three independent experiments. The values of injected groups were compared with those of uninjected controls by one-way ANOVA followed by the Duncan test. *P and #P < 0.05; **P and ##P < 0.01.
Image published in: Liu K et al. (2011)
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