Fig. 5. Analyses of the distribution of XLAP2ω and XLAP2γ proteins on the inner surface of the nuclear envelope (NE) of Xenopus oocyte by using immunogold labelling and scanning electron microscopy (SEM). Manually isolated nuclei from frog oocytes were transferred onto silicone chips. The inner side of the NE was manually exposed by gently removing the nuclear content and processing for SEM immunogold procedures. XLAP2 was detected with antibodies against XLAP2 followed by Protein A coupled with 10-nm gold particles (yellow dots gold particles associated with immunocomplex bound to XLAP2 protein, NPC nuclear pore complexes). a, b Embryonic XLAP2 proteins localize non-randomly and show a tendency to form clusters and microdomains at the inner nuclear membrane. They also show a tendency to locate longitudinally; this might reflect the shape of the nuclear lamina filaments to which they are initially attached. c Typical cluster of XLAP2 proteins. d Typical region lacking XLAP2 protein. Bars 100 nm (a, b), 50 nm (c, d)
Image published in: Chmielewska M et al. (2011)
Image downloaded from an Open Access article in PubMed Central. © The Author(s) 2011
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