Figure 4. H3K27me3 does not correlate with reversibility of the Xi before and after nuclear transfer. (A) Immunofluorescence of X-GFP female EpiSCs, MEFs and TS cells against H3K27me3. Confocal images of H3K27me3 immunostainings (green) counterstained with DAPI (magenta) show that H3K27me3 is enriched on the Xi of female EpiSCs grown on feeders (93%, n=84), the Xi of MEFs (98%, n=123) and the Xi of TS cells (100%, n=32). Scale bars=10 μm. Images are projected Z-sections. (B) H3K27me3 is maintained on the Xi after nuclear transfer. Immunofluorescence of transplanted female MEFs nuclei against H3K27me3 (green). A high proportion (>48%) of female nuclei retain an H3K27me3-labelled Xi up to 72 h after nuclear transfer (arrowheads). The proportion of nuclei carrying an H3K27me3-labelled Xi is shown. n=number of nuclei. DAPI is shown in magenta. Scale bars=2 μm. Images are single Z-sections.
Image published in: Pasque V et al. (2011)
Copyright © 2011, European Molecular Biology Organization. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license
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