Figure 5. H3 tail acetylation stimulates nucleosome mobilization in a bromodomain dependent manner. The rate of nucleosome movement by 80 nm RSC (A and B), 20 nM SWI/SNF (C and D), and 20 nm ΔBr SWI/SNF (E and F) was determined under the same conditions as in Supplementary Figure S5 by gel shift assay using 10 nM 601–603 dinucleosomes. (A, C and E) The concentration of mobilized dinucleosomes moved versus time was plotted and fitted non-linearly to the Michaelis–Menten equation using Graph Pad Prism. (B, D and F) The rate at which the first H2A/H2B dimer is displaced from dinucleosomes was estimated by tracking the appearance of the remodeled species I in two independent experiments. This rate was determined in the same way as the rate of nucleosome movement.
Image published in: Chatterjee N et al. (2011)
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