Figure 7. Aβ1−42 pores with high open probabilities contribute disproportionately to the total cellular Ca2+ load. The Ca2+ load contributed by a given pore was determined by integrating the area under the fluorescence trace (30-s duration), during which the fluorescence exceeded a threshold of 0.2 ΔF/Fo. (A–F) The abscissa represents the number of pores within a 10 × 10–µm imaging field ranked in order of increasing Ca2+ load and normalized to 100%. (A–C) Data obtained 20 min after application of Aβ1−42 are shown. Plots of pore Po (B) and amplitude of the largest SCCaFT observed at each site (C) on the same abscissa as in A are shown. (D–F) Corresponding analyses from the same membrane region as in A–C obtained 10 min later, showing a marked increase in proportion of pores contributing a high Ca2+ load. Blue bars (righthand ordinate; shown in A) show the Ca2+ load contributed by each individual pore expressed as an integral of the fluorescence signals during all SCCaFTs detected at that site. The curve (left ordinate; red) plots the cumulative contribution of pores toward the normalized total Ca2+ load.
Image published in: Demuro A et al. (2011)
© 2011 Demuro et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license
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