Figure 5. MCI activates MCC gene expression(a–h) Shown is the expression of α-tubulin (a–d) or for FoxJ1 (e–h) in embryos injected with a control morpholino (a,e), with MCI-MOspl (b, f) or with MCI-HGR RNA along with nlacz RNA as a tracer (d,h) or alone as a control (c,g). RNA injected embryos were treated with DEX at stage 11.5, and all embryos were fixed at stage 26, and stained with X-gal (light blue). Scale bar=0.5mm. Insert shows a higher power (i) Animal caps were isolated at stage 10 from embryos injected with ICD RNA, MCI-HGR RNA (MCI), or the two RNAs together, treated with DEX at st11, and extracted for total RNA at stage 12, approximately two hours later. Shown is the log level of expression (±s.d.) relative to ICD values set at zero, as measured in triplicate using QT-PCR and after normalization against a ubiquitously expressed RNA, ODC. (j–m) Single animal blastomeres were injected with MCI-HGR RNA at the two-cell stage. At stage 11, half of the injected and uninjected control embryos were treated with cyclohexemide (CHX) for one hour, and then additionally treated with DEX for another hour to induce MCI-HGR activity. CHX is sufficient to block the onset of α-tubulin expression in the controls (compare k to j), indicating that the CHX treatment is effective, but does not block the activation of α-tubulin by MCI-HGR (m). Scale bar (j)=0.2mm.
Image published in: Stubbs JL et al. (2012)
Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder.
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