Fig. 2. Centrosomal recruitment of Cnn and γ-tubulin is unperturbed in Dgp71WD120 and Dgrip75175 mutant brain cells.(A,B) Images show metaphase 3rd instar larval Ganglion Mother cells (GMCs) from WT, Dgp71WD120, and Dgrip75175 brains that were stained with anti-Cnn (A) (green) or anti-γ-tubulin (B) (red) antibodies; DNA (blue). (C) Images show living metaphase 3rd instar larval NBs from WT, Dgp71WD120, and Dgrip75175 brains expressing γ-tubulin GFP. (D) Quantification of the total amount of centrosomal Cnn, γ-tubulin or γ-tubulin-GFP in brain cells. Centrosomal γ-tubulin or Cnn levels in fixed cells were quantified in at least 7 cells in each of 5 brains. Centrosomal γ-tubulin-GFP levels in living cells were quantified in at least 9 NBs of each genotype. Significance testing was conducted using a Student's t-test. Error bars are S.E.M. Scale bar represents 2.5 µm (A,B) and 5 µm (C).
Image published in: Reschen RF et al. (2012)
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