Larger Image Fig. 4. IRE1α knockdown specifically inhibits the expression of differentiation marker genes. Whole mount in situ hybridization analyses revealed that the expression of insulin (B) and amylase (D) was not detected in IRE1α MO injected embryos at stage 43 compared with control MO injected embryos (A, C). The white arrows point to the positive staining of insulin and amylase. Image published in: Yuan L et al. (2014) Image downloaded from an Open Access article in PubMed Central. © 2014 by the Journal of Biomedical Research. All rights reserved. Gene Synonyms Species Stage(s) Tissue ins.L ins-a, ins-b, ins1, insulin, insulin I, preproinsulin, xins X. laevis Throughout NF stage 43 pancreas dorsal pancreatic bud amy2a.L X. laevis Throughout NF stage 43 pancreas dorsal pancreatic bud ventral pancreatic bud Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + ern1 MO + NF 43 Fig. 4 B, D Anatomical Phenotype abnormal foregut morphology abnormally coiled small intestine Expression Phenotype absent amy2a.L expression in pancreas absent ins.L expression in pancreas Permanent Image Page Printer Friendly View XB-IMG-129748