Fig. 2. The phenotype of Cdx knockdown embryos. A: Single and triple knockdowns of Cdx1, Cdx2, and Cdx4 give rise to a similar range of phenotypes. Single knockdown embryos were injected with 20 ng each of Cdx1-A morpholino oligo (MO), Cdx2-A MO, or Cdx4-A MOs. Triple knockdown embryos were injected with a 20 ng total of the set-1 Cdx1, Cdx2, Cdx4 MOs. B: The phenotypes of larval stage 41 control embryos and embryos exhibiting type 1, 2, and 3 axial defects produced by the knockdown of Cdx function. C: A bar chart showing the proportions of type1, 2, and 3 axial defect embryos in control uninjected embryos, standard control morpholino injected embryos and embryos injected with combined or single set-1 Cdx or set-2 Cdx translation blocking morpholinos. In each case, the total mass injected is made up of equal quantities of each contributing MO. Total injected quantities and n values for each group are indicated on the chart. D: XcadVP16 mRNA rescues the compound Cdx1, Cdx2, and Cdx4 knockdown phenotype. Embryos were injected with a 20 ng of total of the Cdx1, Cdx2, and Cdx4 MO, together with the indicated quantities of XcadVP16 mRNA and cultured until larval stage 41.
Image published in: Faas L and Isaacs HV (2009)
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