Fig. 2. Expression of Thylacine 1. The distribution of Thylacine 1 was analysed by RNase protection assay. (A) Distribution in Xenopus embryos. Lane 1, undigested probes; lane 2, tRNA control; lanes 3- 11, unfertilised egg, stages 8, 10, 12.5, 14, 17, 20, 23, 34 embryo RNA respectively. Two embryo equivalents of RNA (10 mg) was used in each case. (B) Distribution in adult frog tissues. Lane 1, undigested probes; lane 2, tRNA control; lanes 3-10, intestine, stomach, gall bladder, spleen, skeletal muscle, lung, liver, stage 20 RNA respectively. 5 mg of total RNA was used in each assay. As an internal control a probe for XMax2, which is expressed at constant levels throughout early development and in adult tissues (Tonissen and Krieg, 1994), was included in the Thylacine 1 assay.
Image published in: Sparrow DB et al. (1998)
Copyright © 1998. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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