Figure 3 Xvent-2 mimics BMP-2/4 signaling effects on the BMP-4 promoter. A, co-injection of 500 pg of Xvent-2 RNA stimulates both the individual upstream and intron mutants at a similar extent as observed with BMP-2/4 RNA (see Fig. 2 B). B and C, while co-injections of the +1815 (B) or +1738/+1969 intron mutant (C) with 400 pg of Xvent-2 yield more than 2-fold increase of reporter activity, 400 pg of Xvent-1 has no effect. Co-injection of 1 ng of Xvent-2 (P40) or 1 ng of chordin RNA leads for both of these mutants to a significant decrease of reporter activity, which can be rescued by 400 pg of Xvent-2 RNA. Note also, that the activation obtained with 300 pg of BMP-2 RNA is strongly inhibited by co-injection with 1.3 ng of Xvent-2 (P40) RNA.
Image published in: Schuler-Metz A et al. (2000)
Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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