FIGURE 8. PTB domain of Xdpcp is essential for interaction with Alk4. A, mapping of deletion mutants. B, HEK293FT cells were transfected with HA-tagged Alk4 alone or in combination with each deletion mutant as indicated. Cell lysates were immunoprecipitated (IP) with anti-HA antibody, followed by immunoblotting (IB) with anti-GFP antibody to observe their association. C, luciferase assays in Xenopus embryos injected with combinations of the indicated reagents. The amount of injected reagents is as follows: xnr-1 RNA, 100 pg; RNAs of Xdpcp full-length and deletion mutants, 2 ng; 6DE-Luc reporter DNA, 40 pg. Error bars represent the standard deviation.
Image published in: Cheong SM et al. (2009)
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