Fig. 3. Nkx2.5 is necessary for differentiation of heart and myeloid cells in dorsal marginal zone (DMZ) explants. Embryos were injected with Control morpholino oligos (MO) (18.4 pmol/embryo) (b, e, h) and with Nkx2.5 MO (18.4 pmol/embryo) (c, f, i) into the DMZ region at the 4-cell stage. Intact embryos served as controls (a, d, g). DMZ explants excised from these embryos were cultured until st. 18 and processed for whole-mount in situ hybridization to examine the expression of c/ebpa (a, b, c), spib (d, e, f), and mpo (g, h, i). The number in each panel indicates total number of samples from three independent experiments. As was observed in the experiment using whole embryos, expression of myeloid cell markers was suppressed by injection of Nkx2.5 MO (c, f, i). In contrast, expression of myeloid cell markers was not affected by injection of Control MO (d, e, f).
Image published in: Sakata H and Maéno M (2014)
Copyright © 2014. Image reproduced with permission of the Publisher, John Wiley & Sons.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| cebpa.L | C/EBP, C/EBPalpha, xC/EBP | X. laevis | Throughout NF stage 18 | myeloid cell anterior ventral blood island |
| spib.L | spi-b, spib-a, spib-b | X. laevis | Throughout NF stage 18 | myeloid cell anterior ventral blood island |
| mpo.L | mpo-A, myeloperoxidase, pmr-1, pmr1, pox2, xpox2 | X. laevis | Throughout NF stage 18 | myeloid cell anterior ventral blood island |
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