Fig. 5. Transcription factors expressed in the ventral blood islands (aVBI) cooperate for myeloid cell differentiation in the ventral marginal zone (VMZ) explant. (a) RT-PCR analysis was performed in whole embryo (WE) at st. 25. (b) Designated mRNAs (c/ebpa, 1 ng; nkx2.5, 100 pg; gata4gr, 50 pg) were injected into the VMZ at the 4-cell stage. The VMZ tissues were excised at st. 10.5, and some embryos injected with gata4gr were treated with DEX. The explants were cultured until st. 25 and RT-PCR analysis was performed to detect the expression of mpo and ef1a in VMZ explants injected with designated mRNAs. Expression of mpo was induced by coinjection of c/ebpa and nkx2.5 or by coinjection of c/ebpa and gata4gr. Overexpression of c/ebpa, nkx2.5 and gata4gr induced a maximum expression of mpo.
Image published in: Sakata H and Maéno M (2014)
Copyright © 2014. Image reproduced with permission of the Publisher, John Wiley & Sons.
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