FIG. 2. Programmed cell death as detected by whole-mount TUNEL staining at the neural plate stage. (A) Stage 13, dorsal (top) and lateral (bottom) view. The dorsal side of the embryo is TUNEL stained with a band of staining broadening toward the anterior end of the embryo, delineating the neural plate. (B) Stage 13, dorsal (top) and lateral (bottom) view. Two broad bands of staining extend laterally from the blastopore slit. (C) Stage 13, dorsal (top) and lateral (bottom) view. A broad medial stripe of staining was detected on the dorsal side. (D) Stage 14, lateral view, anterior is left, no TUNEL staining was detected. (E) Stage 15, lateral view, anterior is left. Intense TUNEL staining was detected in the anteriormost portion of the embryo. Light staining was evident posteriorly. (F) Stage 14, dorsal (top) and lateral (bottom) view, anterior is left. A semicircular band of staining in the anteriormost portion of the embryo, corresponding to the anterolateral edge of the neural plate. (G) Stage 15, end on anterior view (top), lateral view (bottom), anterior is left. TUNEL staining formed a horseshoe-shaped band of staining in a more anteroventral position than stage 14. The embryos shown are representative of the staining observed following staining of 58 stage 13 embryos of which 67% were TUNEL positive, and 37 stage 14/15 embryos of which 52% were TUNEL positive. Embryos with more than 5 TUNEL-stained nuclei were considered TUNEL positive.
Image published in: Hensey C and Gautier J (1998)
Copyright © 1998. Image reproduced with permission of the Publisher, Elsevier B. V.
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