Supplement Fig. 9. Wild type and mutant XFrs3 mRNAs are translated with similar efficiencies. Indicated combinations of XFrs3 MO (7.5 pmole/embryo) and C-terminal Flagtagged XFrs3 mRNA (XFrs3-Flag, 50 pg each/embryo) were injected to equatorial region of 2-cell embryos. The levels of XFrs3-Flag proteins in stage 10 embryos are compared by anti- Flag immunoblotting [Flag (XFrs3)]. Actin is used as a loading control.
Image published in: Kim YJ et al. (2015)
Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V.
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