Fig. 5. Hnf1b but not osr2 and lhx1 expression is inhibited in the KF [kidney field] of Pax8 morphant at neurula stage. (A) In situ hybridization for lhx1, osr2 and hnf1b genes. Embryos were injected with MoPax8 at the 4-cell stage, in the equatorial region of both left blastomeres. At neurula stage 18, they were fixed, transversely bisected at the level of the pronephric area and analyzed by in situ hybridization with the indicated probes. For each embryo, the dotted line indicates the frontier between the injected side (on the left) and the uninjected side (on the right). Hnf1b expression is inhibited in the pronephric area (arrow) on the injected side. (B) RT-qPCR analysis of lhx1, osr2 and hnf1b gene expression in KF explants dissected at neurula stage 18 from MoC and MoPax8 injected embryos. Unlike lhx1 and osr2 expression which is not modified, hnf1b expression is downregulated in response to Pax8 depletion. Average values from four independent experiments. *P<0.05.
Image published in: Buisson I et al. (2015)
Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| lhx1.L | lim-1, Lim1, lim1, Xlim-1, Xlim1 | X. laevis | Throughout NF stage 18 | neural tube mesoderm lateral plate mesoderm axial mesoderm |
| osr2.S | osr2-a, osr2-b, xosr2 | X. laevis | Throughout NF stage 18 | mesoderm lateral plate mesoderm dorsal lateral plate mesoderm |
| hnf1b.L | hnf-1beta, hnf1-beta, hnf1beta, lfb3, tcf2, vhnf1 | X. laevis | Throughout NF stage 18 | endoderm axial mesoderm lateral plate mesoderm dorsal lateral plate mesoderm |
Image source: Published
| Experiment + Assay | Source | Phenotypes and Disease | ||
|---|---|---|---|---|
| Xla Wt + pax8 MO + NF18 (in situ hybridization) | Fig. 5 A c1r3, B c1r3 |
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