Fig. 7. Overexpression of Xdsh mRNA leads to neuralization of animal cap explants. Animal caps from embryos injected with 1 ng of Xdsh RNA were explanted from the injected embryos at stage 8 and cultured in isolation until the equivalent of stage 31 (A) or stage 11 (B). After culture, the explant RNA was extracted and analyzed by northern blotting using different 32P-labeled antisense RNA probes. RNA from ten animal cap equivalents or from two embryos is loaded per lane. The same blot was stripped and reprobed with different probes. Two major specific transcripts of NCAM are shown (Kintner and Melton, 1987). EF1a and fibronectin probes are controls for loading. The muscle-specific actin probe weekly cross-hybridizes with cytoskeletal actin RNAs (the two bands above muscle-specific band). Lane 1, animal caps from embryos injected with 1 ng of Xdsh mRNA; lane 2, animal caps from embryos injected with 0.12 ng of noggin mRNA; lane 3, control animal caps from uninjected embryos; lane 4, sibling embryos, stage 31 (A) or 11 (B).
Image published in: Sokol SY et al. (1995)
Copyright © 1995. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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