Figure 10. Whole-mount in situ hybridization assays at early stages illustrating common expression patterns of 10 down-regulated genes: IMAGE 4174145, IMAGE 3399268, IMAGE 4057931, IMAGE 3400511, Socs3, MGC114680, IMAGE 7010138, Cnfn1-a, Ralgds, Dnaja4.2. For all genes, expression is enhanced in the animal cells (an) of cleavage and blastula embryos, and is throughout the ectoderm at gastrula stages (side views). At early neural plate stages, all genes are expressed in the epidermis (epi), often more intensely on the dorsal (d) side, including neural plate (np) and border zone (bz) (all except IMAGE 3400511 are side views with anterior [a] to the left; IMAGE 3400511 is an anterior view). At later neural plate and neural tube stages, expression is strongest in the neural crest (nc), pre-placodal region (ppr), neural tube (nt) and epidermis (epi) (anterior views). Cnfn1-a is uniquely expressed in the hatching gland (hg).
Image published in: Yan B et al. (2015)
Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| septin5.S | sep1, sept5, septin 1 | X. laevis | Sometime during NF stage 10 to NF stage 20 | neural plate neural tube animal hemisphere |
| dnaja4.2.S | X. laevis | Sometime during NF stage 10 to NF stage 20 | animal hemisphere | |
| cnfn.1.L | cnfn.1-a, cnfn.1-b, cornifelin, plac8l2 | X. laevis | Sometime during NF stage 10 to NF stage 20 | animal hemisphere hatching gland hatching gland primordium |
| clcnkb.L | ClC-k, clc-kb, clckb, clcnka, clk-k2, xClC-k | X. laevis | Sometime during NF stage 10 to NF stage 20 | neural plate animal hemisphere |
| socs3.L | atod4, cis3, cish3, socs-3, ssi-3, ssi3, xsocs3 | X. laevis | Sometime during NF stage 10 to NF stage 20 | neural plate animal hemisphere |
| ralgds.S | X. laevis | Sometime during NF stage 10 to NF stage 20 | neural plate preplacodal ectoderm anterior placodal area animal hemisphere | |
| fam89a.L | X. laevis | Sometime during NF stage 10 to NF stage 20 | animal hemisphere |
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