Fig. 6. Kdm4a overexpression compensated for the effects of PRDM12 via demethylation of H3K9 on the neural crest genes promoter. (A) RT-PCR analysis of neural crest marker expression in the animal cap cells injected with Wnt8 and Chordin mRNAs (each at 50 pg/embryo), with or without PRDM12 and Kdm4a mRNA. (B, C) Luciferase assays on embryos injected with pFoxd3-2000-luc (30 pg/embryo), pGL-TK (10 pg/embryo), and Kdm4a mRNA (0, 50, 100, 200 pg/Sembryo) into the dorsal side at the four-cell stage. Protein was prepared from the injected embryos at stage 18. Error bars indicate ±S.D. (n=3). (D–G) ChIP-qPCR using an anti-H3K9me3 antibody was performed with the Chd and Wnt8 mRNA-injected animal caps with or without injection of PRDM12 mRNA or PRDM12 and Kdm4a mRNAs. The Six1 promoter was used as a negative control. Error bars indicate ±S.E. (n=3).
Image published in: Matsukawa S et al. (2015)
Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V.
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