Figure 3. SL1 expression in the prospective heart region of tailbud embryos. RNA from anterior-dorsal (D) and anterior-ventral (V) fragments of embryos was analyzed by RNase protection assay for the presence of cardiac actin, XMLC2, SL-1, and SL-2 transcripts (A-D, respectively). The equivalent of five fragments was used in each assay. Samples were also tested for the presence of a myotomal muscle-specific marker (labeled "XMHC") (Logan and Mohun 1993), detected using a probe for XMHCa RNA (E). Explants were dissected from late neurula (stage 18) to late tailbud (stage 28) embryos.
Image published in: Chambers AE et al. (1994)
Copyright © 1994. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article.
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