Figure 3. Analysis of Xnot expression by RNase protection. (A) RNA was isolated from five unfertilized eggs and five embryos each at different stages of development. RNA was analyzed by RNase protection using a mixture of Xnot and EFI-a probes, with the EFI-a probe at a reduced specific activity. Note that the MBT occurs at 7 hr and that 10 hr corresponds to a stage 10 (early gastrula) embryo. Stage 12 is late gastrula stage, stage 15 is neural plate stage, stage 19 is neural tube stage, and stage 24 is tailbud stage. (B) Distribution of Xnot in a stage 9 (late blastula) embryo. Stage 9 embryos were dissected into animal (An), middle {Mid), and vegetal (Veg) thirds and analyzed as in A.
Image published in: von Dassow G et al. (1993)
Copyright © 1993. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article.
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