FIGURE 3:. Chromosomal passenger complex delocalization in prometaphase in cells expressing mutant Survivin-myc proteins and depleted of endogenous Survivin by siRNA treatment. (A) HeLa cells stably expressing vector alone or Survivin-myc containing the indicated mutations were transfected with siRNA as shown, synchronized by double thymidine block and then released into fresh medium for 12 h, followed by immunostaining with anti–myc epitope antibody (green), anti–Aurora B antibody (red), and ACA. Scale bar, 5 μm. (B) For cells treated as in A, Aurora B localization classified by immunofluorescence microscopy in ∼100 cells in each condition is shown on the left. Means ± SD (n = 3). Right, the ratio of centromere to chromosome arm fluorescence intensity for Aurora B and Survivin-myc. Means ± SD (n = 5). ***p < 0.001, **p < 0.01, *p < 0.05 compared with cells expressing Survivin-myc WT by one-way analysis of variance followed by Bonferroni's multiple comparison test. ND, not determined.
Image published in: Niedzialkowska E et al. (2012)
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