Figure 7. ephrinB2 and flotillin-1 morphants show neural tube closure defects(a) Neural tube closure defects in ephrinB2 or flotillin-1 morphants. Dorsal view of stage 18 control, ephrinB2, flotillin-1a or flotillin-1b MO-injected embryos, and those co-injected with morpholino-resistant RNA (B211MT-HA for B2MO, F1aΔUTR-Flag for F1aMO and F1bΔUTR-Flag for F1bMO) Left is anterior, and right is posterior. (b) Quantification of neural tube closure defects in ephrinB2 or flotillin-1 morphants. Embryos showing a single dorsal line were counted as having closed neural tubes. At least three independent experiments were performed and error bars indicate standard deviation (s.d.) (c) ephrinB2, flotillin-1a or flotillin-1b MOs were injected into embryos, and neural tube closure was examined at neurula (st. 18/19) and swimming tadpole stages. Note the anterior neural tube defects. (d) Percentage of neural tube closure in ephrinB2 MO, flotillin-1a MO or flotillin-1b MO-injected embryos. Note that the percentage of neural tube closure is somewhat elevated at later stages in B2MO, F1aMO and F1bMO-injected embryos, indicating that some of the embryos displayed a profound delay in neural tube closure, while others retain an open neural tube. Error bars indicate s. d. (e) ephrinB1 MO or flotillin-2 MO injected embryos show normal neural tube closure.
Image published in: Ji YJ et al. (2014)
Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder.
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