Fig. 4. Time course of TALEN-mCherry fusion protein expression in oocytes and embryos. (A,D) Schematic representation of the Tyr-TALEN-mCherry (A) and Tyr-TALEN-mCherry-DS (D) mRNAs. (B,E) Oocytes were injected with either Tyr-TALEN-mCherry (B) or Tyr-TALEN-mCherry-DS (E) mRNAs, transferred into a host female, and fertilized. Five oocytes or host-transferred embryos were homogenized at the indicated time and examined by Western blot analysis using anti-DsRed and anti-actin antibodies. The closed and open arrowheads indicate the positions of the Tyr-TALEN-mCherry and actin proteins, respectively. The stage 13 embryos in the right-most lane were not injected with mRNAs. (C,F) Quantification of the Tyr-TALEN-mCherry protein signals shown in (B,E). The Tyr-TALEN-mCherry proteins were normalized to actin and the signal for the lysates of the stage 13-injected embryos was set to 100%. P, progesterone-treatment.
Image published in: Nakajima K and Yaoita Y (2015)
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