Fig. S8. Expression profile of different FGF receptors in Xenopus embryos. A. Total RNA was extracted from early neurula stage embryos, followed by Lightcycler qPCR (27 cycles) with each receptor-specific primer set, and amplification products were visualized by gel electrophoresis. B. Expression profile of FGF receptors in morphant gastrula stage embryos by qPCR. Samples were prepared and analyzed as per Fig. 5. Relative fgfr expression levels were calculated from the “crossing point” (CP) of qPCR cycle numbers for each primer set, using a common standard curve generated from fgfr1IIIb control embryo cDNA dilution series, and normalized to levels of odc transcripts. Bar graphs indicate the relative expression level of receptor transcripts in embryos injected with 150 ng control (CT), 100 ng pqbp1 (PQ), 50 ng wbp11 (BP), and combined pqbp1 and wbp11 (PQ+BP) MOs. Mean values for triplicate biological experiments are plotted; bars indicate standard error. Note, although expression levels of fgfr1IIIc and fgfr3IIIb transcripts appear nearly zero, they were detected in gelbased and qPCR.
Image published in: Iwasaki Y and Thomsen GH (2014)
Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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