FIGURE 2. Expression of PIAS2b and XCtBP during early Xenopus development. A, RNase protection analysis of PIAS2b mRNA. Total RNA from 10 oocytes at the indicated Dumont stage (85) was hybridized with an antisense 32P-labeled RNA probe. The sample was then digested with RNase One, analyzed on a denaturing polyacrylamide gel, and detected by autoradiography. B, Western blot analysis of PIAS2b. Protein extracts (2 oocyte or 1.5 embryo equivalents) were prepared from developmentally staged oocytes and embryos (86) as indicated, including progesterone-matured oocytes (M) and unfertilized eggs (E). The position of molecular weight standards (in kilodaltons) is indicated. C, Western blot analysis of XCtBP.
Image published in: Malik MQ et al. (2014)
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