Figure S4. TGF- signaling did not impact on the expression of major regulators of ciliogenesis at the GRP, Related to Figure 4. Foxj1 (St. 11.5), RFX2 (St. 14/15) and TTC25 (St. 14/15) expression in nodal1/gdf3 double-depleted embryos, analyzed by WISH. The Foxj1-stained embryos are shown in vegetal view at stage 11.5, RFX2 as well as TTC25 staining are shown as dorsal explants of GRPs. Black or red arrowheads indicate normal expression of these genes and reduction of TTC25 expression, respectively. A white triangle indicates the central region of the GRP. a: anterior, p: posterior, L: left, R: right. “n” indicates number of embryos analyzed.
Image published in: Tözser J et al. (2015)
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