Figure 7. Velocity sedimentation of P216L and C214S peripherin-2 mutants under nonreducing conditions. COS-1 cells expressing bovine P216L (A) and C214S mutant (B) were solubilized in Triton X-100 and subjected to velocity sedimentation. Western blots of the fractions run on nonreducing SDS gels were labeled with the Per2B6 peripherin-2 antibody. The P216L peripherin-2 exhibits a profile consisting of noncovalent tetramers (a) and higher order disulfide-linked oligomers (b) similar to that observed for WT peripherin-2 (Loewen and Molday, 2000). The C214S peripherin-2 produces a mixture of noncovalent dimers (c), tetramers composed of disulfide-linked dimers (d), and aggregated species that sediment near the bottom of the tube.
Image published in: Loewen CJ et al. (2003)
Copyright © 2003. Image reproduced with permission of the Publisher, American Society for Cell Biology (ASCB). This is an Open Access article.
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