Fig. 7. Activation of PI3K partially rescues eye development in kermit 2/XGIPC-depleted embryos. (A) p110*, a constitutively active subunit of PI3 kinase, induces AKT phosphorylation/activation in stage 10 animal cap explants. mRNA encoding p110* was injected into fertilized eggs and animal caps were explanted at the gastrula stage and analyzed by western blot with antibodies recognizing AKT phosphorylated at serine-473 (upper panel) or general anti-AKT antibodies (lower panel). (B) p110* partially rescues eye development in kermit 2-depleted embryos. Kermit 2 morpholino (40 ng), 3 ng of p110* mRNA, or both, were injected into two dorsal animal blastomeres of four-cell/eight-cell embryos. No apparent eyes are present in embryos injected with the kermit 2 morpholino, while small eyes are present in over 70% of embryos co-injected with p110* mRNA and morpholino. p110* injection alone does not affect embryo development. (C) The percentage of embryos with normal, small or absent/miniscule eyes is summarized. (D) p110* partially recovers the expression of Xrx in kermit 2-depleted embryos. One dorsal-animal blastomere of four-cell/eight-cell embryos was injected with kermit 2 morpholino with or without p110* mRNA (right side, indicated by GFP lineage tracer) and harvested at stage 20. Whole-mount in situ hybridization was performed for Xrx. Embryos are viewed from the anterior side with dorsal towards the top.
Image published in: Wu J et al. (2006)
Copyright © 2006. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| rax.S | rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A | X. laevis | Throughout NF stage 20 | eye primordium |
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