Fig. 5. Mutations in the KKR or PPEY motif result in an enlarged notochord with many vacuoles. (A) Whole embryos are shown for the point mutants at stage 32. Immunodetection of laminin (a-c) and with the Tor70 antibody (g-i) at the stages indicated in the top right. The arrowhead in b points the laminin (red) present around the cell. Arrowheads in h and i indicate numerous vacuoles. (d-f) In situ hybridization for the chordin gene. (B) Histogram showing the number of Hoechst-stained nuclei in ten transverse sections in the truncal part of wild-type and mutant embryos at stages 15 and 32. Results are means from five independent experiments. (C) Frontal sections of mutants at the level of the notochord. Four-cell-stage embryos were injected with ras-GFP mRNA alone (control; green), or with ras-GFP mRNA and mutant mRNAs into the right blastomeres. RLDx (red) was injected into the left blastomeres. In controls (a,c), red and green cells periodically intercalate and extend along the notochord width (arrowheads in a,c). In mutants, the regular succession of ras-GFP cells (green, arrowheads in b,d) and RLDx cells (red) is lost. Dashed lines outline the notochord. Scale bars: 500 μm in A upper panels; 25 µm in Aa-i,Ca-d.
Image published in: Buisson N et al. (2014)
Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| lama1.S | LOC108718546 | X. laevis | Throughout NF stage 15 | extracellular matrix |
| chrd.S | chd, chordin, chrd.1, LOC108716939, X-chordin | X. laevis | Throughout NF stage 15 | notochord |
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